©2020 Biological and Chemical Oceanography Data Management Office.The data contain individual respiration rate measurements for developmental stages of Acartia tonsa, collected from eastern Long Island Sound in July 2021. Individuals were exposed to either ambient or increased temperature as nauplii, and then tracked through development. At each developmental stage, respiration rate was measured for the individual using a PreSens respirometer. High resolution data on individual respiration rates through development are crucial for understanding how climate cha...
Show moreAcartia tonsa individuals were collected in July from Esker Point, Connecticut in Eastern Long Island Sound in July of 2021. Laboratory cultures were established and maintained at 18°C, with a 12:12 light:dark cycle. To initiate the experiment, nauplii were collected from the laboratory culture and split into two groups, which were either maintained under rearing conditions or exposed 28°C for 24 hours. For the remaining duration of the experiment, all individuals were held at 18°C in individual petri dishes and fed ad libitum a mixture of Tetraselmis sp. and Rhodomonas salina.
Individual copepods were then tracked through development by examining the petri dish daily for the presence of a molted exoskeleton. Respiration rates were measured for each individual starting in the first copepodite stage, with proceeding measurements during each successive stage. During these measurements, individuals were isolated in 2 ml glass vials with an oxygen sensor spot attached to the base. Oxygen levels were measured every three minutes using a Presens sensor dish reader. Assays typically ran for approximately eight hours. Individual lengths were measured using an inverted compound microscope and attached digital camera, and then individuals were returned to their petri dish with fresh medium. Upon reaching maturity (the sixth copepodite stage), respiration and body size was measured as above and then individual sex was recorded.
In addition to the individuals measured at every stage, a group of individuals had measurements made only in the sixth and final stage, serving as a handling control group to test for effects of repeated handling on respiration or body size. The entire experiment (comprising naupliar heat stress, individual measurements through development, and handling control measurements) was repeated three separate times.
Organism identifiers (Life Science Identifier (LSID))
copepod, Acartia tonsa, urn:lsid:marinespecies.org:taxname:345943
feeding medium, Rhodomonas salina, urn:lsid:marinespecies.org:taxname:106316
feeding medium, Tetraselmis sp., urn:lsid:marinespecies.org:taxname:134526
Holmes-Hackerd, M., Sasaki, M., Dam, H. G. (2025) Individual respiration rate measurements for developmental stages of Acartia tonsa during temperature experiments with copepods collected from eastern Long Island Sound in July of 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-03-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.955729.1 [access date]
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