Dataset: Energy reserve and stable isotope data from 3 species of Australian coral exposed to increased temperature and nutrients treatment

Daily temperature was recorded using Hobo temperature loggers every minute within the treatment tank. Chl a, total soluble lipid, soluble animal protein, and soluble animal carbohydrate concentrations, were each measured on a 1cm² cored plugs of M. monasteriata and from 1cm long branch tips of A. formosa and P. damicornis from each ramet. Each measurement was made on whole coral samples (skeleton, animal tissue, and endosymbiotic algae) that were ground with a mortar and pestle and normalized to total ash-free dry tissue biomass of the organic fraction (animal tissue and endosymbiotic algae). Chl a was extracted using methods modified from Jeffrey and Humphrey (1975). Total soluble lipids were extracted using methods described in Rodrigues and Grottoli (2007), while soluble animal carbohydrate and protein concentrations were measured using the methods modified from Dubois et al. (1956) and Smith et al. (1985), respectively, as described in Levas et al. (2018). Biomass was measured according to methods outlined in McLachlan et al. (2020). 

Coral fragments were airbrushed to remove all tissue from the skeleton. The host tissue and endosymbionts were separated by centrifugation and filtered onto prebaked GF/F filters. Animal host tissue and endosymbiotic algal fraction δ¹⁵N values (δ¹⁵N_h and δ¹⁵N_e, respectively) were reported relative to air (δ¹⁵N = per mil deviation of the ratio of stable nitrogen isotopes ¹⁵N:¹⁴N relative to air). Animal host tissue and endosymbiotic algal fraction δ¹³C values (δ¹³C_h and δ¹³C_e, respectively) were reported relative to Vienna Peedee Belemnite Limestone standard (δ¹³C = per mil deviation of the ratio of stable carbon isotopes ¹³C:¹²C relative to V-PDB). Repeated measurements of internal standards (n = 20) had a standard deviation of ± 0.14‰ for organic δ¹⁵N and ± 0.07‰ for organic δ¹³C.  δ¹⁵N and δ¹³C values were determined using a Costech Elemental Analyzer where the resulting N₂ and CO₂ gases were analyzed for δ¹⁵N and δ¹³C with a ThermoFisher Delta IV stable isotope ratio mass spectrometer (IRMS) via a Conflo II interface in the Grottoli lab at the Ohio State University.