©2020 Biological and Chemical Oceanography Data Management Office.Metagenomic sequence data from Lau Basin hydrothermal vent mollusc gill tissues that contain chemosynthetic bacterial symbionts. Samples were collected between May 2009 to April 2022 using the ROV Jason aboard R/V Thompson cruises TN235 (May-June 2009) and TN401 (April 2016) and R/V Falkor cruise FK160407 (April 2022) for population and comparative genomics analysis of host animal and bacterial symbionts.
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Samples were collected between May 2009 to April 2022 aboard R/V Thompson cruises TN235 (May-June 2009) and TN401 (April 2016) and R/V Falkor cruise FK160407 (April 2022) using the ROV Jason.
Mollusc samples were collected with remotely operated vehicles from hydrothermal vent fields along the Eastern Lau Spreading Center-Valu Fa Ridge and Tonga Volcanic Arc. On board the ship, the symbiont-containing gill tissue of each animal was excised and either flash-frozen or stored in RNALater™ (Thermo Fisher Scientific, Inc., Waltham, MA, USA) at –80°C. DNA was extracted with the E.Z.N.A Mollusc DNA Kit (Omega Bio-tek, Norcross, GA) at the University of Rhode Island and sent to Psomagen, Inc. (Rockville, MD, USA) for metagenomic library preparation and sequencing. Libraries were prepared with the plexWell384 and Twist 96-Plex kits and 150 bp paired-end sequenced on Illumina NovaSeq 6000 and NovaSeq X Plus instruments.
Breusing, C., Beinart, R. A. (2025) NCBI Metagenomic metadata for Lau Basin (Tonga) mollusc gill tissue collected on R/V Thompson cruises TN235 and TN401 and R/V Falkor cruise FK160407 between May 2009 and Apr 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-08-06 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.964236.1 [access date]
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This dataset is licensed under Creative Commons Attribution 4.0.
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