Dataset: Incomplete assimilatory nitrate reduction by LLI Prochlorococcus in response to nutrient stress.

Name: Incomplete assimilatory nitrate reduction by LLI Prochlorococcus in response to nutrient stress.
Published: 2025-08-06
Keywords: Prochlorococcus, nitrate assimilation, primary nitrite maximum, chemostat, nitrite, nitrogen limitation, oceans

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.969427.1Version 1 (2025-08-06)Dataset Type:experimental

Principal Investigator, Contact: Paul Berube (Massachusetts Institute of Technology)

Co-Principal Investigator: Sallie W. Chisholm (Massachusetts Institute of Technology)

Technician: Trent LeMaster (Massachusetts Institute of Technology)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)

Project: Features and implications of nitrogen assimilation trait variability in populations of Prochlorococcus (Prochlorococcus N assimilation)

Abstract

These data include Prochlorococcus cell density, nitrate (NO3-) and nitrite (NO2-) concentration, and fluorometry data for experiments that examined nitrite production by LLI Prochlorococcus following a pulse of nitrate under stress conditions. The conditions evaluated included nitrogen limitation.

Cite Dataset

23

Views

35

Downloads

These numbers come from Google Analytics and reflect real user activity on the site. They reliably show page usage and are mostly free of bot traffic.

This dataset is part of a larger study on the impact of different physiological states/responses of Prochlorococcus on nitrite production potential in the presence of nitrate. The different perturbations yielding the different physiological states/responses and the respective analyses are listed below. Links to data for each perturbation can be found in the Related Datasets section.

The analyses include:

Cell concentration (Nitrogen limitation dataset, Light perturbation dataset, Temperature perturbation dataset)
Nitrite concentration (Nitrogen limitation dataset, Light perturbation dataset, Temperature perturbation dataset)
Nitrate + nitrite concentration (Nitrogen limitation dataset)
Bead standard normalized forward scatter (Nitrogen limitation dataset)
Bead standard normalized red (chlorophyll) fluorescence (Nitrogen limitation dataset)
FIRe fluorometry (Light perturbation dataset, Temperature perturbation dataset)

See Related Datasets section below for links to above mentioned datasets.

Related Datasets

IsRelatedTo

Dataset: Temperature and light perturbations
Relationship Description: Light and temperature shock dataset as part of same experiment.

Berube, P., Chisholm, S. W. (2025) Incomplete assimilatory nitrate reduction by LLI Prochlorococcus in response to light and temperature stress. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-07-31 doi:10.26008/1912/bco-dmo.969513.1

Massachusetts Institute of Technology. Continuous cultures of nitrogen-limited Prochlorococcus. 2025/05. In: BioProject [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; 2011-. Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA1262978. NCBI:BioProject: PRJNA1262978

Related Publications

Results

Berube, P. M., LeMaster, T., & Chisholm, S. W. (2025). Priming the pump: Enhanced nitrite release in response to a nitrate pulse by nitrogen-limited Prochlorococcus. https://doi.org/10.1101/2025.05.13.653865

Methods

Berube, P. M., Biller, S. J., Kent, A. G., Berta-Thompson, J. W., Roggensack, S. E., Roache-Johnson, K. H., Ackerman, M., Moore, L. R., Meisel, J. D., Sher, D., Thompson, L. R., Campbell, L., Martiny, A. C., & Chisholm, S. W. (2014). Physiology and evolution of nitrate acquisition in Prochlorococcus. The ISME Journal, 9(5), 1195–1207. https://doi.org/10.1038/ismej.2014.211

Methods

Moore, L. R., Coe, A., Zinser, E. R., Saito, M. A., Sullivan, M. B., Lindell, D., Frois-Moniz, K., Waterbury, J., & Chisholm, S. W. (2007). Culturing the marine cyanobacterium Prochlorococcus. Limnology and Oceanography: Methods, 5(10), 353–362. Portico. https://doi.org/10.4319/lom.2007.5.353

Loading...Still loading...Hang on... This is taking longer than expected!

Dataset: Incomplete assimilatory nitrate reduction by LLI Prochlorococcus in response to nutrient stress.

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.969427.1Version 1 (2025-08-06)Dataset Type:experimental

Principal Investigator, Contact: Paul Berube (Massachusetts Institute of Technology)

Co-Principal Investigator: Sallie W. Chisholm (Massachusetts Institute of Technology)

Technician: Trent LeMaster (Massachusetts Institute of Technology)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)

Project: Features and implications of nitrogen assimilation trait variability in populations of Prochlorococcus (Prochlorococcus N assimilation)

Abstract

Cite Dataset

23

Views

35

Downloads

These numbers come from Google Analytics and reflect real user activity on the site. They reliably show page usage and are mostly free of bot traffic.

The analyses include:

Cell concentration (Nitrogen limitation dataset, Light perturbation dataset, Temperature perturbation dataset)
Nitrite concentration (Nitrogen limitation dataset, Light perturbation dataset, Temperature perturbation dataset)
Nitrate + nitrite concentration (Nitrogen limitation dataset)
Bead standard normalized forward scatter (Nitrogen limitation dataset)
Bead standard normalized red (chlorophyll) fluorescence (Nitrogen limitation dataset)
FIRe fluorometry (Light perturbation dataset, Temperature perturbation dataset)

See Related Datasets section below for links to above mentioned datasets.

Related Datasets

IsRelatedTo

Dataset: Temperature and light perturbations
Relationship Description: Light and temperature shock dataset as part of same experiment.

Related Publications

Results

Methods

Data File(s)	Type	Description	Action
969427_v1_chemostat.csv (72.21 KB)	Comma Separated Values (.csv)	Primary data file for dataset ID 969427, version 1	Add to Cart 969427_v1_chemostat.csv View Table

Supplemental File(s)	Type	Description	Action
flow_cytometry_chemostat_cultures.tar.gz (104.80 MB)	GZIP (.gz)	This file contains archived and compressed raw flow cytometry data files (FCS3 format) for the chemostat cultures experiment. File names match those provided in the primary data file.	Add to Cart flow_cytometry_chemostat_cultures.tar.gz

Data File(s)	Type	Description	Action
969427_v1_chemostat.csv (72.21 KB)	Comma Separated Values (.csv)	Primary data file for dataset ID 969427, version 1	Add to Cart 969427_v1_chemostat.csv View Table

Supplemental File(s)	Type	Description	Action
flow_cytometry_chemostat_cultures.tar.gz (104.80 MB)	GZIP (.gz)	This file contains archived and compressed raw flow cytometry data files (FCS3 format) for the chemostat cultures experiment. File names match those provided in the primary data file.	Add to Cart flow_cytometry_chemostat_cultures.tar.gz

Dataset: Incomplete assimilatory nitrate reduction by LLI Prochlorococcus in response to nutrient stress.

Principal Investigator, Contact: Paul Berube (Massachusetts Institute of Technology)

Co-Principal Investigator: Sallie W. Chisholm (Massachusetts Institute of Technology)

Technician: Trent LeMaster (Massachusetts Institute of Technology)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)

Project: Features and implications of nitrogen assimilation trait variability in populations of Prochlorococcus (Prochlorococcus N assimilation)

Abstract

23

35

Files

Supplemental Files

Related Datasets

Related Publications

Dataset: Incomplete assimilatory nitrate reduction by LLI Prochlorococcus in response to nutrient stress.

Principal Investigator, Contact: Paul Berube (Massachusetts Institute of Technology)

Co-Principal Investigator: Sallie W. Chisholm (Massachusetts Institute of Technology)

Technician: Trent LeMaster (Massachusetts Institute of Technology)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)

Project: Features and implications of nitrogen assimilation trait variability in populations of Prochlorococcus (Prochlorococcus N assimilation)

Abstract

23

35

Files

Supplemental Files

Related Datasets

Related Publications

Dataset: Incomplete assimilatory nitrate reduction by LLI Prochlorococcus in response to nutrient stress.

Principal Investigator, Contact: Paul Berube (Massachusetts Institute of Technology)

Co-Principal Investigator: Sallie W. Chisholm (Massachusetts Institute of Technology)

Technician: Trent LeMaster (Massachusetts Institute of Technology)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)

Project: Features and implications of nitrogen assimilation trait variability in populations of Prochlorococcus (Prochlorococcus N assimilation)

Abstract

Metadata

23

35

Files

Supplemental Files

Related Datasets

Related Publications

Dataset: Incomplete assimilatory nitrate reduction by LLI Prochlorococcus in response to nutrient stress.

Principal Investigator, Contact: Paul Berube (Massachusetts Institute of Technology)

Co-Principal Investigator: Sallie W. Chisholm (Massachusetts Institute of Technology)

Technician: Trent LeMaster (Massachusetts Institute of Technology)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)

Project: Features and implications of nitrogen assimilation trait variability in populations of Prochlorococcus (Prochlorococcus N assimilation)

Abstract

Metadata

23

35

Files

Supplemental Files

Related Datasets

Related Publications