©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2020 Biological and Chemical Oceanography Data Management Office.Bacterial abundance was measured on glutaraldehyde-fixed seawater samples collected from nutrient amendment experiments conducted on surface water collected at the Long-Term Ecological Monitoring Station, MareChiara, in the Gulf of Naples, Italy, between April 20 and May 19, 2022. Bacteria were counted using Sybr Green I staining and flow cytometry in whole seawater (free and particle-associated bacteria) and 3-micron filtered seawater (free bacteria). These incubations were part of a field stud...
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Samples were collected at Station MareChiara (40°49'N, 14°15'E), Gulf of Naples, Italy. Whole seawater (whole) or seawater filtered through a 3-micrometer (um) polycarbonate filter (<3 um) is transferred to a cryovial containing a final concentration of glutarldehyde of 0.5%. The sample is stored at 4 degrees Celsius (C) for 30 minutes, then flash frozen in liquid nitrogen and stored at -80 degrees C. Upon analysis, samples were thawed, and a final concentration of 10 micrograms per milliliter (ug/ml) of Tween-80 was added. Samples were sonicated at 50 watts (W) in a water bath for 1 minute. Samples were added to 1X TE buffer (0.01 molar (M) Tris, pH 8.0, 5 micromolar (uM) EDTA) with Sybr Gold (1:20,000 dilution) and heated at 80 degrees C for 10 minutes in the dark. Samples were cooled to room temperature for ~5 minutes before analyzing on an Accuri C6 Plus flow cytometer. Bacteria were gated based on SybrGold fluorescence and side scatter.
Thamatrakoln, K., Bidle, K. D., Kranzler, C. F. (2025) Bacterial abundance collected from project "DaVINCi" incubations in the Gulf of Naples, Italy from April to May 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-07-31 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/970493 [access date]
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