Dataset: Peroxide quantification in T. testudinum tissue collected [month?, year?] from seagrass beds in Tampa Bay, Florida

Provided by PI:
To quantify the levels of internal H2O2, a Peroxide Assay Kit was utilized according to the manufacturer’s protocol (Sigma-Aldrich). Peroxide concentration was calculated as picomoles of H2O2 per μg of protein.

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Example text suggested by BCO-DMO:

A colorimetric hydrogen peroxide assay was conducted on the seagrass Thalassia testudinum to assess ______ [?oxidative stress of seagrass?? Or impact of Labyrinthula?]. Hydrogen peroxide (H₂O₂) standards (labeled A–H) were prepared at known concentrations of 0, 3, 6, 9, 12, 18, 24, and 30 micromolar [?is this correct?] using ______ water [was it Milli-Q or other ultrapure water?] and hydrogen peroxide [what percentage or molarity? What brand/type?]. A Sigma Aldrich Peroxide Assay Kit was used and the absorbance at 585 nm was measured for both standards and samples. The assay uses the chromogenic Fe3+-xylenol orange reaction (FOX), in which a purple complex is formed when Fe2+ is oxidized to Fe3+ by peroxides present in the sample, generating a colorimetric (585 nm) result, proportional to the level of peroxide present (Sigma-Aldrich, 2019; from Peroxide Assay Kit (MAK311) Technical Bulletin). Sample extracts were treated and put into a 96-well [or 48 or other?] plate for analysis with a Biotek Synergy Plate Reader.