Chlorophyll a was measured on seawater samples collected from nutrient amendment incubation experiments conducted on surface water collected at the Long-Term Ecological Monitoring Station, MareChiara, in the Gulf of Naples, Italy, between April 20 and May 19, 2022.
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Samples from incubation bottle treatments were collected after gently mixing treatment bags and siphoning water into a bottle used for filtering. Chlorophyll a was measured by filtering triplicate samples of 50-100 milliliters (mL) of water through 25-millimeter (mm) glass fiber filters (GFF, Whatman) using vacuum filtration. Filters were then extracted in 5 mL of 91% ethanol for at least 12 hours in the dark at room temperature in glass test tubes. Following extraction, the relative fluorescence of each sample was read on a Turner Designs handheld fluorometer (model 8000-010) and then acidified with 2 drops of 10% hydrochloric acid and read again on the fluorometer to correct for phaeophytin (Graff & Rynearson, 2011). Chlorophyll a standards purchased from Turner were used to calibrate the fluorometer prior to measurement.
Thamatrakoln, K., Harvey, E. (2025) Chlorophyll a concentrations collected from Project "DaVINCi" incubations in the Gulf of Naples, Italy from April to May 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-09-17 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/984494 [access date]
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