Dataset: Measurements of C–P lyase phosphate thresholds in Stutzerimonas frequens cultures (North Pacific Methane Project)

Cultures of S. frequens were grown in 150 mL of seawater medium in acid-clean, sterilized glass serum bottles. The medium was amended with methylphosphonate to a final concentration of 73.5 nM, and the fluorescently labeled phosphonate tracer n-DPPh (Naphthyl-diphenylphosphonate) was added to the medium to a final concentration of 7.4 nM. Cultures were incubated for 4 days.

At the end of incubation, samples were collected for chemical and cellular analyses. 10 mL of headspace gas were withdrawn via an airtight syringe for methane concentration and δ¹³C isotope analysis using a Picarro gas analyzer. 50 mL of culture medium were collected for phosphorus analysis using standard colormetric methods (Murphy et al.,1962). 1 mL of medium was collected for cell counts via flow cytometry. Finally, 10 mL of medium were collected for C-P lyase activity analysis by HPLC coupled with a fluorescence detector.