Dataset: C and N stable isotope data for invertebrates collected by HOV Alvin during R/V Atlantis cruise AT50-24 at Sanak Seep Alaska from May 16 to June 5, 2024

Pushcore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator. Rocks were placed into individual compartments in a biobox on the Alvin basket. Before preserving, samples were kept cold and animals were handpicked to sample tissue for stable isotope analyses. The tissue (aprox. 0.5-2.5 mg) was placed in pre-weighted tin boats or combusted vials (500C overnight) and frozen at -70C.

In addition to natural abundance analyses, some animals were placed into mylar bags with addition of labeled C and N and protected from oxygen and light for incubation experiments. The bags were kept sealed in the ship's cold room for 3-8 days, and animals were then processed following the same method as for natural abundance samples.

 Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters. Filters were frozen for further processing in the laboratory.