Dataset: Sequence accession numbers for metatranscriptomic sequences from coral microbiomes experimentally exposed to low oxygen in 2022

Coral colonies were collected in the field from two sites (Cayo Roldan and Hospital Point), fragmented into smaller samples, and acclimated in aquaria for seven days. Ten 40-L aquaria were set up for ex situ manipulation of dissolved oxygen. Five tanks were bubbled with nitrogen gas to induce hypoxic conditions. Five tanks were maintained as normoxic conditions. During the two-week treatment period, corals in hypoxia tanks were exposed to low oxygen conditions (0.3 mg/L dissolved oxygen) for 6 hours each night. Samples were collected after the two-week treatment period and after a two-week recovery period with no induced low oxygen conditions. Whole fragments were preserved in RNAlater and frozen until processing at the University of Florida. RNA extractions were performed with the Qiagen RNeasy Mini Kit with the addition of bashing beads. RNAseq libraries were constructed with the NuGEN Universal Prokaryotic RNA-Seq, Prokaryotic AnyDeplete kit. Libraries were submitted to the University of Florida Interdisciplinary Center for Biotechnology Research (RRID:SCR_019152) for sequencing on an Illumina NovaSeq6000 with paired 150-bp reads.