Water samples were collected for DNA extraction using sampling bottles deployed on the CTD rosette. At each depth, 2L samples were filtered through 0.22 µm filters (Millipore, Darmstadt, Germany) using a peristaltic pump and frozen until extraction. DNA was extracted following Beman et al. (2012) and sent for metagenome sequencing in the Vincent J. Coates Genome Sequencing Laboratory (GSL) at the University of California, Berkeley. For each sample, 250 ng of genomic DNA was sheared and librari...
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Water samples were collected for DNA extraction using sampling bottles deployed on the CTD rosette. At each depth, 2-liter (L) samples were filtered through 0.22-micrometer (µm) filters (Millipore, Darmstadt, Germany) using a peristaltic pump and frozen until extraction. DNA was extracted following Beman et al. (2012) and sent for metagenome sequencing in the Vincent J. Coates Genome Sequencing Laboratory (GSL) at the University of California, Berkeley. For each sample, 250 ng of genomic DNA was sheared and libraries were prepared using the KAPA HyperPrep Kit (Kapa Biosystems, Wilmington, MA, USA). Samples were pooled into a single lane and sequenced via 150- cycle paired-end sequencing on the Illumina HiSeq 4000 platform (Illumina, Inc., San Diego, CA, USA).
Beman, J. M. (2025) Metagenome accession numbers for samples collected in the eastern tropical North Pacific Ocean in 2017 on R/V Oceanus cruise 1704A. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-10-27 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/986605 [access date]
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This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.