Dataset: NCBI accession metadata for microbial community responses to bacterial extracellular vesicles in the Sargasso Sea, collected on R/V Atlantic Explorer cruises AE2412 and AE2427 in Apr and Nov 2024

Community samples were collected using Niskin bottles over a period of four days aboard the R/V Atlantic Explorer, during cruises AE2412 and AE2427, April 25-28 and November 20-23, 2024 from the Bermuda Atlantic Time-series Study (BATS) site in the Sargasso Sea (31.66 N 64.16 W).

Replicate 250 mL polycarbonate bottles (A, B, and C) were filled with 200 mL of surface seawater collected in the Sargasso Sea. Each bottle received either 100 ul of 1x PBS buffer (control), 0.01% glucose (final w/v), or purified extracellular vesicles (approximately 1e9 EVs/mL final concentration). Replicates were placed in a deckboard flow-through incubator and sampled after 0, 24, 48, and 72 hours. The microbial community was sampled by filtering water through a 47mm 0.2 µm Supor filter and frozen at -80 C.  DNA was extracted using a phenol-chloroform method utilizing AMPureXP beads (Biller et al 2018). 16S amplicon generation (V4-V5 primers) and sequencing on an Illumina MiSeq was conducted at the Integrated Microbiome Resource, Dalhousie University.