This dataset includes particulate triacylglycerol lipid data from samples collected on three research cruises in 2021 and 2023. In the Gulf of Maine, samples were collected on R/V Endeavor (cruise EN667) from 13 June to 21 June, 2021. Off the Oregon Coast, samples were on the R/V Sally Ride (cruise SR2310) from 18 May to 25 May, 2023. In the Sargasso Sea, samples were collected on R/V Atlantic Explorer (cruise AE2320) from 5 September to 11 September, 2023. Lipid samples were collected via CTD r...
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Methods on EN667:
One liter (L) samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47-millimeter (mm) 0.2-micrometer (um) Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.
Lipid samples were processed and analyzed according to Holm et al. (2022), and quantified via Holm et al. (2024). Per-liter particulate lipid values are reported in peak area and femtomoles per liter. All values for all compounds were blank-corrected using the average of five 1-L filtered seawater blanks collected from the sea surface on EN667.
Methods on SR2320:
One liter samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47 mm 0.2 um Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.
Lipid samples were processed and analyzed according to Holm et al. (2022), and quantified via Holm et al. (2024). Per-liter particulate lipid values are reported in peak area and femtomoles per liter. All values for all compounds were blank-corrected using the average of twenty 0.2 um Durapore filters processed and analyzed alongside sample filters.
Methods on AE2320:
Two liter samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47 mm 0.2 um Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.
Lipid samples were processed and analyzed according to Holm et al. (2022), and quantified via Holm et al. (2024). Per-liter particulate lipid values are reported in peak area and femtomoles per liter. All values for all compounds were blank-corrected using the average of seven 2-L filtered seawater blanks collected from the surface on AE2320.
Lowenstein, D., Van Mooy, B. A. (2026). Particulate triacylglycerol lipid data from cruises EN667 in the Gulf of Maine in June 2021, SR2310 along the Oregon Coast in May 2023, and AE2320 in the Sargasso Sea in September 2023 (RIPPLE1, RIPPLE2, and RIPPLE3). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-05-11 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/997821 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.