Dataset: Particulate organic carbon data from cruises EN667 in the Gulf of Maine in June 2021, SR2310 along the Oregon Coast in May 2023, and AE2320 in the Sargasso Sea in September 2023 (RIPPLE1, RIPPLE2, and RIPPLE3)

Methods on EN667:
One liter (L) samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47-millimeter (mm) 0.2-micrometer (um) Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.

Particulate organic carbon (POC) samples were fumed for 24 hours with hydrochloric acid to remove inorganic carbon, then analyzed on an isotope ratio mass spectrometer. POC samples were blank corrected using the average of three 1 liter filtered seawater blanks filtered through 0.7 um GF/F filters from the surface on EN667.

Methods on SR2320:
One liter samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47 mm 0.2 um Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.

POC samples were fumed for 24 hours with hydrochloric acid to remove inorganic carbon, then analyzed on an isotope ratio mass spectrometer. POC samples were blank corrected using the average of four 1 L filtered seawater blanks filtered through 0.7 um GF/F filters from the surface on SR2310.

Methods on AE2320:
Two liter samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47 mm 0.2 um Durapore filters (lipids, carbs; Millipore) and 25mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.

POC samples were fumed for 24 hours with hydrochloric acid to remove inorganic carbon, then analyzed on an isotope ratio mass spectrometer. POC samples were blank corrected using the average of three 2 L filtered seawater blanks filtered through 0.7 um GF/F filters from the surface on AE2320.